People typically don’t mind being asked for money, as long as you aren’t pushy about it. If you’ve connected with someone and they have the tools and information that they need to make the donation, give it some time before reaching out to them again.
People are busy and get distracted easily, so after a while give them a soft reminder with an update on where you are in relation to your fundraising goal and new developments in MS research.
A simple gesture like that will remind them that they still need to donate.
Finally, send out one last donation request 48 hours before your event to make sure you get those people who are pressure prompted (most of us!).